目的 研究西南獐牙菜(Swertia cincta Burk.) 全株的化学成分及其抗前列腺增生活性,为开发利用提供药理学研究依据。方法 采用正、反相硅胶柱、RP-HPLC、凝胶柱、薄层制备等色谱技术,通过波谱分析进行结构鉴定;采用皮下注射丙酸睾酮建立SD(Sprague-Dawley)大鼠前列腺增生模型,观察各组前列腺指数的变化。结果 从西南獐牙菜全株中分离到 11个化合物,经波谱数据分析分别鉴定为獐牙菜苦苷(1),(R)-(-)-龙胆苦苷(2),獐牙菜苷(3),6′-O-β-D-葡萄糖基獐牙菜苷(4),6′-O-β-D-葡萄糖基龙胆苦苷(5),马钱苷酸(6),齐墩果酸(7),2α,3β-dihydroxyolean-12-en-28-oic acid-28-O-β-D-glucopyranoside(8),β-胡萝卜苷(9),4-hydroxyl-3,5-dimethoxyl-6-O-β-D-glucosebenzene(10), 异红草苷(11),其中獐牙菜苦苷能显著性降低前列腺指数。结论 化合物5和8为首次从獐牙菜属植物中分离得到;其中化合物1具有一定的抗前列腺增生作用。
Abstract
OBJECTIVE To study the chemical constituents extracted from the whole plants of Swertia cincta Burk. and their antagonistic effects on benign prostatic hyperplasia(BPH). METHODS The compounds were isolated by various chromatographic methods including silica gel, reverse silica gel, RP-HPLC, Sephadex LH-20, preparative TLC, and so on. Their structures were identified by extensive analysis of the spectroscopic data. Experimental prostatic hyperplasia was produced in male Sprague-Dawley(SD) rats by testosterone injection for 28d, and then the prostate index(PI) was measured.RESULTS Eleven compounds were isolated from the whole plants of Swertia cincta Burk. and identified as swertiamarin(1),(R)-(-)-gentiopicroside(2), sweroside(3), 6′-O-β-D-glucopyranosylsweroside(4), 6′-O-β-D-glucopyranosylgentiopicroside(5), loganic acid(6), oleanolic acid(7), 2α,3β-dihydroxyolean-12-en-28-oicacid-28-O-β-D-glucopyranoside(8), β-daucosterol(9), 4-hydroxyl-3,5-dimethoxyl-6-O-β-D-glucosebenzene(10), and isoorientin(11). Swertiamarin significantly decreased PI in SD rats. CONCLUSION Compounds 5 and 8 were isolated from the plants of Swertia cincta for the first time. Compound 1 has favorable effect for inhibiting prostatic hyperplasia in SD rats.
关键词
西南獐牙菜 /
獐牙菜苦苷 /
6′-O-β-D-葡萄糖基-龙胆苦苷 /
前列腺增生
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Key words
Swertia cincta Burk. /
swertiamarin /
6′-O-β-D-glucopyranosylgentiopicroside /
benign prostatic hyperplasia
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金资助项目(21262048)
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